|
|
| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
02/03/2017 |
Actualizado : |
07/03/2017 |
Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
Idioma : |
Inglés |
Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 02512naa a2200217 a 4500 001 1056750 005 2017-03-07 008 2015 bl uuuu u00u1 u #d 100 1 $aGARCIA, J.P 245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource] 260 $c2015 520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aFINNIE, J.W. 700 1 $aMANAVIS, J. . 700 1 $aBEINGESSER, J. 700 1 $aADAMS ,V. 700 1 $aROOD, J.I. 700 1 $aUZAL, F.A. 773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
19/01/2017 |
Tipo de producción científica : |
Serie Técnica |
Autor : |
CORSI, W.; GENTA, H. |
Afiliación : |
WALTER CORSI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Asesor Agroclimatología (INIA Salto Grande), Dirección General de Servicios Agronómicos. MGAP.; HECTOR GENTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Asesor Horticultura (INIA Salto Grande). |
Título : |
Heladas en el área hortifrutícola de Salto |
Fecha de publicación : |
1992 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 1992. |
Páginas : |
29p |
Serie : |
(INIA Serie Técnica; 22) |
ISBN : |
9974-556-28-7 |
ISSN : |
1688-9266 |
Idioma : |
Español |
Contenido : |
El objetivo del presente trabajo es presentar información de utilidad a los productores del área de Salto para mejorar su conocimiento del régimen de heladas de la región. La Estación Experimental INIA-Salto Grande, ha realizado durante los últimos 20 años un relevamiento de las temperaturas de congelamiento ocurridas, para conocer sus características en la región y de esta manera poner a disposición del productor elementos para poder defenderse mejor de esta adversidad climática. Esta información permite tomar medidas más apropiadas de manejo en los cultivos, como poder planificar sus operaciones con anticipación para hacer un uso más eficiente del período libre de heladas. También es propósito de este trabajo presentar conceptos generales sobre las heladas y su caracterización. |
Thesagro : |
DAÑOS POR LA HELADA; FRUTICULTURA; HELADA; HORTICULTURA; RESISTENCIA A LA TEMPERATURA; RESISTENCIA A LAS HELADAS; URUGUAY. |
Asunto categoría : |
-- P40 Meteorología y climatología |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/2925/1/111219220807120050.pdf
|
Marc : |
LEADER 01457nam a2200253 a 4500 001 1002721 005 2017-01-19 008 1992 bl uuuu u00u1 u #d 020 $a9974-556-28-7 022 $a1688-9266 100 1 $aCORSI, W. 245 $aHeladas en el área hortifrutícola de Salto 260 $aMontevideo (Uruguay): INIA$c1992 300 $a29p 490 $a(INIA Serie Técnica; 22) 520 $aEl objetivo del presente trabajo es presentar información de utilidad a los productores del área de Salto para mejorar su conocimiento del régimen de heladas de la región. La Estación Experimental INIA-Salto Grande, ha realizado durante los últimos 20 años un relevamiento de las temperaturas de congelamiento ocurridas, para conocer sus características en la región y de esta manera poner a disposición del productor elementos para poder defenderse mejor de esta adversidad climática. Esta información permite tomar medidas más apropiadas de manejo en los cultivos, como poder planificar sus operaciones con anticipación para hacer un uso más eficiente del período libre de heladas. También es propósito de este trabajo presentar conceptos generales sobre las heladas y su caracterización. 650 $aDAÑOS POR LA HELADA 650 $aFRUTICULTURA 650 $aHELADA 650 $aHORTICULTURA 650 $aRESISTENCIA A LA TEMPERATURA 650 $aRESISTENCIA A LAS HELADAS 650 $aURUGUAY 700 1 $aGENTA, H.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|